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single cell wga kit  (Vazyme Biotech Co)


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    Structured Review

    Vazyme Biotech Co single cell wga kit
    Single Cell Wga Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 94/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/single+cell+wga+kit/Discover-sc+Single+Cell+WGA+Kit/pmc12758452-214-30-35
    Average 94 stars, based on 40 article reviews
    single cell wga kit - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: Stage- and tissue-specific gene editing using 4-OHT–inducible Cas9 in whole organism
    Article Snippet: .. For the detection of mutation efficiency within this system, PGCs and SCs of zebrafish embryos were collected via fluorescence-activated cell sorting (FACS) and subjected to whole-genome amplification (WGA) using the Single Cell WGA Kit (N603; Vazyme). ..

    Article Title: Stage- and tissue-specific gene editing using 4-OHT-inducible Cas9 in whole organism.
    Article Snippet: .. For the detection of mutation efficiency within this system, PGCs and SCs of zebrafish embryos were collected via fluorescenceactivated cell sorting (FACS) and subjected to whole-genome amplification (WGA) using the Single Cell WGA Kit (N603; Vazyme). ..

    Fluorescence:

    Article Title: Stage- and tissue-specific gene editing using 4-OHT–inducible Cas9 in whole organism
    Article Snippet: .. For the detection of mutation efficiency within this system, PGCs and SCs of zebrafish embryos were collected via fluorescence-activated cell sorting (FACS) and subjected to whole-genome amplification (WGA) using the Single Cell WGA Kit (N603; Vazyme). ..

    FACS:

    Article Title: Stage- and tissue-specific gene editing using 4-OHT–inducible Cas9 in whole organism
    Article Snippet: .. For the detection of mutation efficiency within this system, PGCs and SCs of zebrafish embryos were collected via fluorescence-activated cell sorting (FACS) and subjected to whole-genome amplification (WGA) using the Single Cell WGA Kit (N603; Vazyme). ..

    Article Title: Stage- and tissue-specific gene editing using 4-OHT-inducible Cas9 in whole organism.
    Article Snippet: .. For the detection of mutation efficiency within this system, PGCs and SCs of zebrafish embryos were collected via fluorescenceactivated cell sorting (FACS) and subjected to whole-genome amplification (WGA) using the Single Cell WGA Kit (N603; Vazyme). ..

    Article Title: Stage- and tissue-specific gene editing using 4-OHT-inducible Cas9 in whole organism.
    Article Snippet: .. For validation of off-targeting effects within this system, PGCs of zebrafish embryos were also collected via FACS and subjected to WGA using the Single Cell WGA Kit (N603; Vazyme), followed by genomic DNA library preparation using the DNA Library Prep Kit for Illumina (TD503; Vazyme). ..

    Article Title: Stage- and tissue-specific gene editing using 4-OHT–inducible Cas9 in whole organism
    Article Snippet: .. For validation of off-targeting effects within this system, PGCs of zebrafish embryos were also collected via FACS and subjected to WGA using the Single Cell WGA Kit (N603; Vazyme), followed by genomic DNA library preparation using the DNA Library Prep Kit for Illumina (TD503; Vazyme). ..

    Whole Genome Amplification:

    Article Title: Stage- and tissue-specific gene editing using 4-OHT–inducible Cas9 in whole organism
    Article Snippet: .. For the detection of mutation efficiency within this system, PGCs and SCs of zebrafish embryos were collected via fluorescence-activated cell sorting (FACS) and subjected to whole-genome amplification (WGA) using the Single Cell WGA Kit (N603; Vazyme). ..

    Article Title: Stage- and tissue-specific gene editing using 4-OHT-inducible Cas9 in whole organism.
    Article Snippet: .. For the detection of mutation efficiency within this system, PGCs and SCs of zebrafish embryos were collected via fluorescenceactivated cell sorting (FACS) and subjected to whole-genome amplification (WGA) using the Single Cell WGA Kit (N603; Vazyme). ..

    Article Title: Stage- and tissue-specific gene editing using 4-OHT-inducible Cas9 in whole organism.
    Article Snippet: .. For validation of off-targeting effects within this system, PGCs of zebrafish embryos were also collected via FACS and subjected to WGA using the Single Cell WGA Kit (N603; Vazyme), followed by genomic DNA library preparation using the DNA Library Prep Kit for Illumina (TD503; Vazyme). ..

    Article Title: Stage- and tissue-specific gene editing using 4-OHT–inducible Cas9 in whole organism
    Article Snippet: .. For validation of off-targeting effects within this system, PGCs of zebrafish embryos were also collected via FACS and subjected to WGA using the Single Cell WGA Kit (N603; Vazyme), followed by genomic DNA library preparation using the DNA Library Prep Kit for Illumina (TD503; Vazyme). ..

    Article Title: Stage- and tissue-specific gene editing using 4-OHT–inducible Cas9 in whole organism
    Article Snippet: .. The genomes of PGC mixtures, individual PGCs, and individual mouse blastomeres were extracted and amplified with the Single Cell WGA Kit (N603; Vazyme), diluted with ddH 2 O to a suitable concentration, and used as PCR templates. summarizes the primer sequences for genotyping. .. When detecting the target gene mutations in HEK293T cells, embryos, and cell mixtures, PCR products were further examined by T7EI (NEB) digestion at 37°C for 30 min, followed by sequencing for determination of the mutated sequence.

    Article Title: Characterization of the oral microbiota of Kawasaki disease patients by metagenomic analysis: A pilot study.
    Article Snippet: DNA concentrations were determined using the Qubit dsDNA HS (high sensitivity) Assay Kit (Thermo Fisher Scientific). .. The Single Cell WGA Kit (Vazyme) was used to amplify the DNA if the concentration was less than 1 ng/μL. .. Third, libraries were prepared with the Nextera XT DNA Library Prep Kit and sequenced using the NextSeq 550 system (150-bp paired-end reads, Illumina).

    Article Title: Stage- and tissue-specific gene editing using 4-OHT-inducible Cas9 in whole organism.
    Article Snippet: .. The genomes of PGC mixtures, individual PGCs, and individual mouse blastomeres were extracted and amplified with the Single Cell WGA Kit (N603; Vazyme), diluted with ddH2O to a suitable concentration, and used as PCR templates. ..

    Article Title: Stage- and tissue-specific gene editing using 4-OHT inducible Cas9 in whole organism
    Article Snippet: .. The genomes of PGC mixtures, individual PGCs, and individual mouse blastomeres were extracted and amplified with the Single Cell WGA Kit (Vazyme, N603), diluted with ddH 2 O to a suitable concentration, and used as PCR templates. ..

    Single Cell:

    Article Title: Stage- and tissue-specific gene editing using 4-OHT–inducible Cas9 in whole organism
    Article Snippet: .. For the detection of mutation efficiency within this system, PGCs and SCs of zebrafish embryos were collected via fluorescence-activated cell sorting (FACS) and subjected to whole-genome amplification (WGA) using the Single Cell WGA Kit (N603; Vazyme). ..

    Article Title: Stage- and tissue-specific gene editing using 4-OHT-inducible Cas9 in whole organism.
    Article Snippet: .. For the detection of mutation efficiency within this system, PGCs and SCs of zebrafish embryos were collected via fluorescenceactivated cell sorting (FACS) and subjected to whole-genome amplification (WGA) using the Single Cell WGA Kit (N603; Vazyme). ..

    Article Title: Stage- and tissue-specific gene editing using 4-OHT-inducible Cas9 in whole organism.
    Article Snippet: .. For validation of off-targeting effects within this system, PGCs of zebrafish embryos were also collected via FACS and subjected to WGA using the Single Cell WGA Kit (N603; Vazyme), followed by genomic DNA library preparation using the DNA Library Prep Kit for Illumina (TD503; Vazyme). ..

    Article Title: Stage- and tissue-specific gene editing using 4-OHT–inducible Cas9 in whole organism
    Article Snippet: .. For validation of off-targeting effects within this system, PGCs of zebrafish embryos were also collected via FACS and subjected to WGA using the Single Cell WGA Kit (N603; Vazyme), followed by genomic DNA library preparation using the DNA Library Prep Kit for Illumina (TD503; Vazyme). ..

    Article Title: Stage- and tissue-specific gene editing using 4-OHT–inducible Cas9 in whole organism
    Article Snippet: .. The genomes of PGC mixtures, individual PGCs, and individual mouse blastomeres were extracted and amplified with the Single Cell WGA Kit (N603; Vazyme), diluted with ddH 2 O to a suitable concentration, and used as PCR templates. summarizes the primer sequences for genotyping. .. When detecting the target gene mutations in HEK293T cells, embryos, and cell mixtures, PCR products were further examined by T7EI (NEB) digestion at 37°C for 30 min, followed by sequencing for determination of the mutated sequence.

    Article Title: Characterization of the oral microbiota of Kawasaki disease patients by metagenomic analysis: A pilot study.
    Article Snippet: DNA concentrations were determined using the Qubit dsDNA HS (high sensitivity) Assay Kit (Thermo Fisher Scientific). .. The Single Cell WGA Kit (Vazyme) was used to amplify the DNA if the concentration was less than 1 ng/μL. .. Third, libraries were prepared with the Nextera XT DNA Library Prep Kit and sequenced using the NextSeq 550 system (150-bp paired-end reads, Illumina).

    Article Title: Stage- and tissue-specific gene editing using 4-OHT-inducible Cas9 in whole organism.
    Article Snippet: .. The genomes of PGC mixtures, individual PGCs, and individual mouse blastomeres were extracted and amplified with the Single Cell WGA Kit (N603; Vazyme), diluted with ddH2O to a suitable concentration, and used as PCR templates. ..

    Article Title: Stage- and tissue-specific gene editing using 4-OHT inducible Cas9 in whole organism
    Article Snippet: .. The genomes of PGC mixtures, individual PGCs, and individual mouse blastomeres were extracted and amplified with the Single Cell WGA Kit (Vazyme, N603), diluted with ddH 2 O to a suitable concentration, and used as PCR templates. ..

    Biomarker Discovery:

    Article Title: Stage- and tissue-specific gene editing using 4-OHT-inducible Cas9 in whole organism.
    Article Snippet: .. For validation of off-targeting effects within this system, PGCs of zebrafish embryos were also collected via FACS and subjected to WGA using the Single Cell WGA Kit (N603; Vazyme), followed by genomic DNA library preparation using the DNA Library Prep Kit for Illumina (TD503; Vazyme). ..

    Article Title: Stage- and tissue-specific gene editing using 4-OHT–inducible Cas9 in whole organism
    Article Snippet: .. For validation of off-targeting effects within this system, PGCs of zebrafish embryos were also collected via FACS and subjected to WGA using the Single Cell WGA Kit (N603; Vazyme), followed by genomic DNA library preparation using the DNA Library Prep Kit for Illumina (TD503; Vazyme). ..

    DNA Library Preparation:

    Article Title: Stage- and tissue-specific gene editing using 4-OHT-inducible Cas9 in whole organism.
    Article Snippet: .. For validation of off-targeting effects within this system, PGCs of zebrafish embryos were also collected via FACS and subjected to WGA using the Single Cell WGA Kit (N603; Vazyme), followed by genomic DNA library preparation using the DNA Library Prep Kit for Illumina (TD503; Vazyme). ..

    Article Title: Stage- and tissue-specific gene editing using 4-OHT–inducible Cas9 in whole organism
    Article Snippet: .. For validation of off-targeting effects within this system, PGCs of zebrafish embryos were also collected via FACS and subjected to WGA using the Single Cell WGA Kit (N603; Vazyme), followed by genomic DNA library preparation using the DNA Library Prep Kit for Illumina (TD503; Vazyme). ..

    Pyrolysis Gas Chromatography:

    Article Title: Stage- and tissue-specific gene editing using 4-OHT–inducible Cas9 in whole organism
    Article Snippet: .. The genomes of PGC mixtures, individual PGCs, and individual mouse blastomeres were extracted and amplified with the Single Cell WGA Kit (N603; Vazyme), diluted with ddH 2 O to a suitable concentration, and used as PCR templates. summarizes the primer sequences for genotyping. .. When detecting the target gene mutations in HEK293T cells, embryos, and cell mixtures, PCR products were further examined by T7EI (NEB) digestion at 37°C for 30 min, followed by sequencing for determination of the mutated sequence.

    Article Title: Stage- and tissue-specific gene editing using 4-OHT-inducible Cas9 in whole organism.
    Article Snippet: .. The genomes of PGC mixtures, individual PGCs, and individual mouse blastomeres were extracted and amplified with the Single Cell WGA Kit (N603; Vazyme), diluted with ddH2O to a suitable concentration, and used as PCR templates. ..

    Article Title: Stage- and tissue-specific gene editing using 4-OHT inducible Cas9 in whole organism
    Article Snippet: .. The genomes of PGC mixtures, individual PGCs, and individual mouse blastomeres were extracted and amplified with the Single Cell WGA Kit (Vazyme, N603), diluted with ddH 2 O to a suitable concentration, and used as PCR templates. ..

    Amplification:

    Article Title: Stage- and tissue-specific gene editing using 4-OHT–inducible Cas9 in whole organism
    Article Snippet: .. The genomes of PGC mixtures, individual PGCs, and individual mouse blastomeres were extracted and amplified with the Single Cell WGA Kit (N603; Vazyme), diluted with ddH 2 O to a suitable concentration, and used as PCR templates. summarizes the primer sequences for genotyping. .. When detecting the target gene mutations in HEK293T cells, embryos, and cell mixtures, PCR products were further examined by T7EI (NEB) digestion at 37°C for 30 min, followed by sequencing for determination of the mutated sequence.

    Article Title: Stage- and tissue-specific gene editing using 4-OHT-inducible Cas9 in whole organism.
    Article Snippet: .. The genomes of PGC mixtures, individual PGCs, and individual mouse blastomeres were extracted and amplified with the Single Cell WGA Kit (N603; Vazyme), diluted with ddH2O to a suitable concentration, and used as PCR templates. ..

    Article Title: Stage- and tissue-specific gene editing using 4-OHT inducible Cas9 in whole organism
    Article Snippet: .. The genomes of PGC mixtures, individual PGCs, and individual mouse blastomeres were extracted and amplified with the Single Cell WGA Kit (Vazyme, N603), diluted with ddH 2 O to a suitable concentration, and used as PCR templates. ..

    Concentration Assay:

    Article Title: Stage- and tissue-specific gene editing using 4-OHT–inducible Cas9 in whole organism
    Article Snippet: .. The genomes of PGC mixtures, individual PGCs, and individual mouse blastomeres were extracted and amplified with the Single Cell WGA Kit (N603; Vazyme), diluted with ddH 2 O to a suitable concentration, and used as PCR templates. summarizes the primer sequences for genotyping. .. When detecting the target gene mutations in HEK293T cells, embryos, and cell mixtures, PCR products were further examined by T7EI (NEB) digestion at 37°C for 30 min, followed by sequencing for determination of the mutated sequence.

    Article Title: Characterization of the oral microbiota of Kawasaki disease patients by metagenomic analysis: A pilot study.
    Article Snippet: DNA concentrations were determined using the Qubit dsDNA HS (high sensitivity) Assay Kit (Thermo Fisher Scientific). .. The Single Cell WGA Kit (Vazyme) was used to amplify the DNA if the concentration was less than 1 ng/μL. .. Third, libraries were prepared with the Nextera XT DNA Library Prep Kit and sequenced using the NextSeq 550 system (150-bp paired-end reads, Illumina).

    Article Title: Stage- and tissue-specific gene editing using 4-OHT-inducible Cas9 in whole organism.
    Article Snippet: .. The genomes of PGC mixtures, individual PGCs, and individual mouse blastomeres were extracted and amplified with the Single Cell WGA Kit (N603; Vazyme), diluted with ddH2O to a suitable concentration, and used as PCR templates. ..

    Article Title: Stage- and tissue-specific gene editing using 4-OHT inducible Cas9 in whole organism
    Article Snippet: .. The genomes of PGC mixtures, individual PGCs, and individual mouse blastomeres were extracted and amplified with the Single Cell WGA Kit (Vazyme, N603), diluted with ddH 2 O to a suitable concentration, and used as PCR templates. ..

    Polymerase Chain Reaction:

    Article Title: Stage- and tissue-specific gene editing using 4-OHT–inducible Cas9 in whole organism
    Article Snippet: .. The genomes of PGC mixtures, individual PGCs, and individual mouse blastomeres were extracted and amplified with the Single Cell WGA Kit (N603; Vazyme), diluted with ddH 2 O to a suitable concentration, and used as PCR templates. summarizes the primer sequences for genotyping. .. When detecting the target gene mutations in HEK293T cells, embryos, and cell mixtures, PCR products were further examined by T7EI (NEB) digestion at 37°C for 30 min, followed by sequencing for determination of the mutated sequence.

    Article Title: Stage- and tissue-specific gene editing using 4-OHT-inducible Cas9 in whole organism.
    Article Snippet: .. The genomes of PGC mixtures, individual PGCs, and individual mouse blastomeres were extracted and amplified with the Single Cell WGA Kit (N603; Vazyme), diluted with ddH2O to a suitable concentration, and used as PCR templates. ..

    Article Title: Stage- and tissue-specific gene editing using 4-OHT inducible Cas9 in whole organism
    Article Snippet: .. The genomes of PGC mixtures, individual PGCs, and individual mouse blastomeres were extracted and amplified with the Single Cell WGA Kit (Vazyme, N603), diluted with ddH 2 O to a suitable concentration, and used as PCR templates. ..



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